A simplified in vitro model for investigation of the antimicrobial efficacy of various antiseptic agents to prevent peri-implantitis
A simplified in vitro model for investigation of the antimicrobial efficacy of various antiseptic agents to prevent peri-implantitis
임플란트 주위염을 예방하기 위한 다양한 소독제의 항균 효능을 조사하기 위한 단순화된 체외 모델
ANNAMARIA VENKEI 1p , GABRIELLA EORDEGH € 2 , KINGA TURZO3 , EDIT URBAN 1 , and KRISZTINA UNGVARI 2 1 Institute of Clinical Microbiology, Faculty of Medicine, University of Szeged, 6725, Semmelweis u. 6, Szeged, Hungary 2 Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, 6720, Tisza Lajos krt. 64, Szeged, Hungary 3 Dentistry Program, Medical School, University of Pecs, 7621, Dischka Gy. u. 5, Pecs, Hungary
The biofilm formation by oral bacteria on the implant surface is one of the most remarkable factors of peri-implant infections, which may eventually lead to bone resorption and loss of the dental implant.
Therefore, the elimination of biofilm is an essential step for the successful therapy of implant-related infections. In this work we created a basic in vitro model to evaluate the antibacterial effect of three widely used antiseptics.
Commercially pure (CP4) titanium sample discs with sand blasted, acid etched, and polished surface were used. The discs were incubated with mono-cultures of Streptococcus mitis and streptococcus salivarius. The adhered bacterial biofilms were treated with different antiseptics: chlorhexidine-digluconate (CHX), povidone-iodine (PI), and chlorine dioxide (CD) for 5 min and the control discs with ultrapure water. The antibacterial effect of the antiseptics was tested by colorimetric assay.
According to the results, the PI and the CD were statistically the most effective in the elimination of the two test bacteria on both titanium surfaces after 5 min treatment time. The CD showed significant effect only against S. salivarius. Based on our results we conclude that PI and CD may be promising antibacterial agents to disinfecting the peri-implant site in the dental practice.
CONCLUSION
In our study we compared the antimicrobial effect of three antiseptics (CHX, PI, CD) on S. mitis and S. salivarius in mono-species biofilm models adhering to Ti surfaces using MTT colorimetric assay. The antimicrobial property of all tested disinfectants have been known, however there are limited information in the literature about their comparison. Our data indicated, that PI and CD had remarkable eliminating property against S. mitis and S. salivarius cells in biofilm after 5 min of treatment time. Considering the conflicts in available literature regarding the cytotoxic properties of agents we established that the PI and CD means advantageous disinfectants, since they are effective in vitro against the biofilms of the pioneer colonizers under aerobic conditions.
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